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RESULTS Between 40 and 65 EOTTD members from 17 European countries and 7 non-European countries attended the clinical workshops held on 6 occasions. Flow diagrams for patient management were composed to display minimum and best practice for most treatment situations. New agreed definitions of recurrence and chemotherapy resistance were formulated. CONCLUSIONS Despite the many differences between and within the participating countries, an important step in uniform treatment of GTD and GTN within Europe was made by the Clinical Working Party of the EOTTD. This is an example on how guidelines and harmonisation can be achieved within international networks. Assembly of novel ecofriendly and sustainable (N-PSPB/SHGL) nanosorbent was fabricated based on encapsulation of derived nanoscale spherical biochar from Pisum sativum pods (N-PSPB) with starch hydrogel (SHGL). The mass ratio between starch and N-PSPB was examined and 2% (w/w) was selected as the optimum percentage for fabrication of the assembled hydrogel. High swelling capacity was characterized by N-PSPB/SHGL nanosorbent (500.0%) at room temperature (25 °C), excellent stability for ten cycles with respect to regeneration by 0.1 mol L-1 HCl. Additionally, characterizations of N-PSPB/Starch nanosorbent were established by SEM and BET measurement to characterize surface area (226.94 m2/g) and pore volume (9.88 cm3/g). The N-PSPB/SHGL nanosorbent was subjected to extensive investigations to evaluate its efficiency for removal of naproxen drug (NAP) and chromium (VI). The Cr(VI) and NAP drug adsorptions were fitted to pseudo-second kinetic and correlated with Langmuir isotherm. The adsorption processes were spontaneous and endothermic based on thermodynamic study. CHARGE syndrome is a rare disease caused by a genetic disorder. The clinical features of this syndrome include coloboma of the eye, heart anomaly, choanal atresia, retardation of mental and somatic development, microphallus, ear abnormalities and/or deafness. CHD7 is the main causative gene for CHARGE syndrome. In this study, we generated an induced pluripotent stem cell (iPSC) line from the dermal fibroblasts of a 1.5-year-old girl, carrying a de novo mutation (CHD7;NM_017780;c.3449_3450delTC;p.L1151Gfs*17). This iPSC line will be a useful tool for investigating the pathogenesis and for developing treatment for this complicated syndrome. This study focused on surface waters from three small creeks, within the Seine River watershed, which are characterized by different land-uses, namely forested, agricultural and urban. Silver nanoparticles (Ag-NPs) in these waters were detected and quantified by single-particle ICPMS during one-year of monthly sampling. Their temporal and spatial variations were investigated. Ag-NPs, in the three types of surface water, were found to range from 1.5 × 107 to 2.3 × 109 particles L-1 and from 0.4 to 28.3 ng L-1 at number and mass concentrations, respectively. These values are in consistent with the very few previous studies. In addition, the role of factors driving process and potential sources are discussed with correlations between Ag-NPs concentrations and biogeochemical parameters, like dissolved organic carbon concentration and divalent cations concentrations. this website For the forested watershed NOM controls the stability (number and mass) of the Ag-NPs as recently observed in the field in lake water in Germany. In ocess of Ag-NPs in natural waters, as well as to the ecotoxicity studies in real-world environment. A new method based on fluorescence spectroscopy for the sensitive determination of aminophylline (AP), an antiasthmatic drug, was developed in this work. Amino-functionalized graphene quantum dots (afGQDs) were synthesized based on a two-step method and they were characterized by transmission electron microscope, UV-vis absorption spectrum and infrared spectrum. The fluorescence of afGQDs was quenched by riboflavin (Rf) via both dynamic quenching and inner filter effect. Photoilluminated Rf-AP system in the presence of oxygen produced hydroxyl radicals (OH). The latter accepted electrons from afGQDs owing to a photo-induced electron transfer process and led to the further fluorescence decline. The changing extent of the fluorescence intensity was found to be proportional to the concentration of AP in the range of 0.10-10 μg mL-1 and the limit of detection arrived at 40 ng mL-1. The proposed method was successfully employed for the determination of AP in a pharmaceutical sample and the recovery rate varied in the range of 99%-106%. An efficient, accurate and sensitive spectrofluorimetric method was developed for analysis of empagliflozin (EGF) in pure form, dosage form and human plasma. The proposed procedure was based on formation of yellow fluorescent product between benzofurazan reagent and empagliflozin in slightly alkaline medium that is measured at 521 nm, when excitation at 455 nm. The present study was validated according to ICH guidelines and bioanalytical validated according to US-FDA guidance. The fluorescence intensity-concentration plot was linear over the range of 50-1000 ng ml-1 with limit of detection (LOD) and quantitation (LOQ) of 15.55 and 46.63 ng ml-1, respectively. The correlation (r) and determination (r2) coefficient was 0.9998 and 0.9997, respectively. Due to high sensitivity and selectivity of the proposed method, it is successfully used for analysis of empagliflozin in its dosage form and human plasma with good recoveries of 98.89% and 98.70%, respectively, without any interfering from matrix components. The corresponding regression equation, Y = 0.756X + 141.93, (r2 = 0.9994) for spiked plasma sample. Two level full factorial designs were used to study different experimental parameters that affect the reaction product and to get the optimum method conditions. The suggested method can be used in quality control lab as well as in pharmacokinetic studies of empagliflozin. To accurately investigate in situ breast cancer would be very significant for real-time information and in situ diagnosis. In this in situ study, home-made hollow optical fiber attenuated total reflection (HOF-ATR) probe was integrated into Fourier transform infrared (FTIR) spectroscopic system to perform breast cancer research at molecular level. Based on the FTIR spectral analysis on band shifts and absorbance ratios, it’s disclosed that the molecular structure, conformation and content of main components change with cancerization of breast tissue. Fisher’s discriminant analysis on HOF-ATR-FTIR spectra was applied to identify the healthy and cancerous breast tissues for the first time. The identification accuracy was 96.67% for training group and 93.33% for cross-validation, respectively, as well as 95% for the prediction group. This paper provides much in situ information of tissue cancerization at molecular level, which can be used as fingerprint biomarkers of tissue cancerization for in situ diagnosis. HOF-ATR-FTIR spectroscopy with discriminant analysis has potential to be an effective and promising method in in situ biomedical research and monitoring of breast cancer.